| Leroy Chiao | Himself (NASA Astronaut) |
| Kenneth D. Cockrell | Himself (NASA Astronaut) |
| Robert L. Curbeam Jr. | Himself (Commander, USN) |
| Brian Duffy | Himself (Colonel, USAF, Ret.) |
| Michael L. Gernhardt | Himself (NASA Astronaut) |
| Yuri Pavlovich Gidzenko | Himself (Lieutenant Colonel, Air Force Russia) |
| Umberto Guidoni | Himself (European Space Agency) |
| Chris A. Hadifield | Himself (Colonel, CAF) |
| Susan J. Helms | Herself (Colonel, USAF) |
| Charles Owen Hobaugh | Himself (Major, USMC) |
| Marsha Ivins | Herself (NASA Astronaut) |
| Thomas D. Jones | Himself (NASA Astronaut) |
| Janet Lynn Kavandi | Herself (NASA Astronaut) |
| James M. Kelly | Himself (Lt. Colonel, USAF) |
| Sergei Krikalev | Himself (Russian Cosmonaut) |
| Steven W. Lindsey | Himself (Lt. Colonel, USAF) |
| Yuri Valentinovich Lonchakov | Himself (Lieutenant Colonel, Russian Air Force) |
| Pamela Ann Melroy | Herself (Lieutenant Colonel, USAF) |
| Mark L. Polansky | Himself (NASA Astronaut) |
Tom Cruise narrates a chronicle of the International Space Station, its construction and the training of its crews.