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| Teresa Wright | Young Charlie Newton |
| Joseph Cotten | Charlie Oakley |
| Hume Cronyn | Herbie Hawkins |
| Macdonald Carey | Jack Graham |
| Henry Travers | Joseph Newton |
| Patricia Collinge | Emma Newton |
| Edna May Wonacott | Ann Newton |
| Wallace Ford | Fred Saunders |
| Irving Bacon | Station Master |
| Charles Bates | Roger Newton |
| Clarence Muse | Railroad Porter |
| Janet Shaw | Louise |
| Minerva Urecal | Mrs. Henderson (clerk at telegraph office) (uncredited) |
| Isabel Randolph | Mrs. Margaret Green (uncredited) |
| Eily Malyon | Librarian (uncredited) |
Uncle Charlie visits his relatives in Santa Rosa. He is a very charming man, but his niece slowly realizes that he is wanted for murder and he soon recognizes her suspicions. Although one of the suspected murderers is killed and the case is considered closed, she still has her suspicions.
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