| Albert Brooks | Marlin |
| Ellen DeGeneres | Dory |
| Alexander Gould | Nemo |
| Willem Dafoe | Gill |
| Brad Garrett | Bloat |
| Allison Janney | Peach |
| Austin Pendleton | Gurgle |
| Stephen Root | Bubbles |
| Vicki Lewis | Deb ( & Flo) |
| Joe Ranft | Jacques |
| Geoffrey Rush | Nigel |
| Andrew Stanton | Crush |
| Elizabeth Perkins | Coral |
| Nicholas Bird | Squirt |
| Bob Peterson | Mr. Ray |
| Barry Humphries | Bruce |
| Eric Bana | Anchor |
| Bruce Spence | Chum |
| Bill Hunter | Dentist |
| LuLu Ebeling | Darla |
| Jordy Ranft | Tad |
| Erica Beck | Pearl |
| Erik Per Sullivan | Sheldon |
| John Ratzenberger | Fish School |
A fish embarks on a journey to find his son after losing him in the Great Barrier Reef.
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