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| Edward G. Robinson | Dr. Paul Ehrlich |
| Ruth Gordon | Mrs. Ehrlich |
| Otto Kruger | Dr. Emil Von Behring |
| Donald Crisp | Minister Althoff |
| Maria Ouspenskaya | Franziska Speyer |
| Montagu Love | Professor Hartmann |
| Sig Rumann | Dr. Hans Wolfert |
| Donald Meek | Mittelmeyer |
| Henry O'Neill | Dr. Lentz |
| Albert Basserman | Dr. Robert Koch |
| Edward Norris | Dr. Morgenroth |
| Harry Davenport | Judge |
| Louis Calhern | Dr. Brockdorf |
| Louis Jean Heydt | Dr. Kunze |
| Charles Halton | Sensenbrenner |
| Irving Bacon | Becker |
| Douglas Wood | Speidler |
| Theodore Von Eltz | Dr. Kraus |
| Hermine Sterler | Miss Marquardt |
| John Hamilton | Hirsch |
| Paul Harvey | Defense Attorney |
| Frank Reicher | Old Man |
| Torben Meyer | Kadereit |
| Louis Arco | Dr. Bertheim |
| Wilfred Hari | Dr. Hata |
| John Henrick | Dr. Bucher |
| Ann Todd | Marianne |
| Polly Stewart | Steffi |
| Egon Brecher | Martl |
| Robert Strange | Koerner |
The German scientist Dr. Paul Ehrlich makes remarkable advancements in the field of medical diagnostics with his research in cellular staining with dyes. His work results in new knowledge about tuberculosis, but also leads to his contraction of the disease. He travels to Egypt, where he investigates diptheria and earns more accolades. Eventually his research in treating diseases leads to the development of a ''magic bullet'' treatment for syphilis.
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