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| Arnold Schwarzenegger | Dr. Victor Fries/Mr. Freeze |
| George Clooney | Bruce Wayne/Batman |
| Chris O'Donnell | Dick Grayson/Robin |
| Uma Thurman | Dr. Pamela Isley/Poison Ivy |
| Alicia Silverstone | Barbara Wilson/Batgirl |
| Michael Gough | Alfred Pennyworth |
| Pat Hingle | Commissioner Gordon |
| John Glover | Dr. Jason Woodrue |
| Elle Macpherson | Julie Madison |
| Vivica A. Fox | Ms. B. Haven |
| Vendela K. Thomessen | Nora Fries |
| Elizabeth Sanders | Gossip Gerty |
| Jeep Swenson | Bane |
Batgirl joins the caped crusaders to stop Mr. Freeze and Poison Ivy from wreaking revenge upon the world.
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