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| Mike Myers | Austin Powers/Dr. Evil/Fat Bastard |
| Heather Graham | Felicity Shagwell |
| Michael York | Basil Exposition |
| Robert Wagner | Number Two |
| Rob Lowe | Young Number Two |
| Seth Green | Scott Evil |
| Mindy Sterling | Frau Farbissina |
| Verne J. Troyer | Mini-Me |
| Elizabeth Hurley | Mrs. Vanessa Kensington-Powers |
| Kristen Johnston | Ivana Humpalot |
| Gia Carides | Robin Spitz Swallows |
| Burt Bacharach | Himself |
| Elvis Costello | Himself |
| Will Ferrell | Mustafa |
| Woody Harrelson | Himself |
| Charles Napier | General Hawk |
| Willie Nelson | Himself |
| Tim Robbins | The President |
| Rebecca Romijn-Stamos | Herself |
| Jerry Springer | Himself |
| Fred Willard | Mission Commander |
The secret agent travels back in time to the 1960s to rescue his stolen mojo from Dr. Evil.
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