| Kenneth Branagh | Narration |
| Glenn Close | Diary Readings |
| Anne Frank | Herself (archive footage) |
| Miep Gies | Herself |
| Edith Frank | Herself (uncredited) (archive footage) |
| Margot Frank | Herself (uncredited) (archive footage) |
| Otto Frank | Himself (uncredited) |
| Johannes Kleiman | Himself (uncredited) (archive footage) |
| Victor Kugler | Himself (uncredited) (archive footage) |
| Fritz Pfeffer | Himself (uncredited) (archive footage) |
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